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Databank Inc cx43 lattice

( A ) A schematic representation of the gap junction assembly from <t>connexin</t> <t>43</t> (Cx) monomers to hemichannels and to the gap junction plaque between two cells. Intra- (IL) and extracellular (EL) loops are labeled. The transmembrane (TM) helices are numbered 1 to 4. ( B ) One-nm-thick slice through a cryo-ET reconstruction of a cell-cell junction. Areas harboring <t>Cx43</t> channels (green), 80 S ribosomes (cyan), and microtubules (pink) have been indicated. A mitochondrion has been labeled (M). Scale bars, 100 nm. ( C ) Close-up of the area indicated in (B). Side views of Cx43 GJCs bridging two plasma membranes are indicated with arrowheads. Scale bar, 10 nm. ( D ) Close-up from a different cryo-ET reconstruction showing top views of the Cx43 GJCs. Scale bar, 10 nm. ( E ) Results of template matching for Cx43 GJCs, 80 S ribosomes, and microtubule segments are shown after plotting the templates back onto the cryo-ET tomogram (illustrated with a 1-nm-thick slice in the background). The gap junction is depicted as a transparent surface (green) fitted to the Cx43 channel positions for visual clarity. ( F ) Histogram of pairwise distances between 80 S ribosomes and microtubule segments (MTs) to the Cx43 GJCs, calculated from multiple cryo-ET reconstructions ( n = 26). The 15-nm-wide zone occupied by Cx43 channels is indicated in green. The 22-nm-wide ribosome exclusion zone (REZ) is indicated in gray.

Cx43 Lattice, supplied by Databank Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cx43 lattice/product/Databank Inc
Average 86 stars, based on 1 article reviews

cx43 lattice - by Bioz Stars, 2026-05

86/100 stars

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1) Product Images from "In situ structure of the human gap junction"

Article Title: In situ structure of the human gap junction

Journal: Science Advances

doi: 10.1126/sciadv.aea4183

( A ) A schematic representation of the gap junction assembly from connexin 43 (Cx) monomers to hemichannels and to the gap junction plaque between two cells. Intra- (IL) and extracellular (EL) loops are labeled. The transmembrane (TM) helices are numbered 1 to 4. ( B ) One-nm-thick slice through a cryo-ET reconstruction of a cell-cell junction. Areas harboring Cx43 channels (green), 80 S ribosomes (cyan), and microtubules (pink) have been indicated. A mitochondrion has been labeled (M). Scale bars, 100 nm. ( C ) Close-up of the area indicated in (B). Side views of Cx43 GJCs bridging two plasma membranes are indicated with arrowheads. Scale bar, 10 nm. ( D ) Close-up from a different cryo-ET reconstruction showing top views of the Cx43 GJCs. Scale bar, 10 nm. ( E ) Results of template matching for Cx43 GJCs, 80 S ribosomes, and microtubule segments are shown after plotting the templates back onto the cryo-ET tomogram (illustrated with a 1-nm-thick slice in the background). The gap junction is depicted as a transparent surface (green) fitted to the Cx43 channel positions for visual clarity. ( F ) Histogram of pairwise distances between 80 S ribosomes and microtubule segments (MTs) to the Cx43 GJCs, calculated from multiple cryo-ET reconstructions ( n = 26). The 15-nm-wide zone occupied by Cx43 channels is indicated in green. The 22-nm-wide ribosome exclusion zone (REZ) is indicated in gray.

Figure Legend Snippet: ( A ) A schematic representation of the gap junction assembly from connexin 43 (Cx) monomers to hemichannels and to the gap junction plaque between two cells. Intra- (IL) and extracellular (EL) loops are labeled. The transmembrane (TM) helices are numbered 1 to 4. ( B ) One-nm-thick slice through a cryo-ET reconstruction of a cell-cell junction. Areas harboring Cx43 channels (green), 80 S ribosomes (cyan), and microtubules (pink) have been indicated. A mitochondrion has been labeled (M). Scale bars, 100 nm. ( C ) Close-up of the area indicated in (B). Side views of Cx43 GJCs bridging two plasma membranes are indicated with arrowheads. Scale bar, 10 nm. ( D ) Close-up from a different cryo-ET reconstruction showing top views of the Cx43 GJCs. Scale bar, 10 nm. ( E ) Results of template matching for Cx43 GJCs, 80 S ribosomes, and microtubule segments are shown after plotting the templates back onto the cryo-ET tomogram (illustrated with a 1-nm-thick slice in the background). The gap junction is depicted as a transparent surface (green) fitted to the Cx43 channel positions for visual clarity. ( F ) Histogram of pairwise distances between 80 S ribosomes and microtubule segments (MTs) to the Cx43 GJCs, calculated from multiple cryo-ET reconstructions ( n = 26). The 15-nm-wide zone occupied by Cx43 channels is indicated in green. The 22-nm-wide ribosome exclusion zone (REZ) is indicated in gray.

Techniques Used: Labeling, Tomography, Clinical Proteomics

( A ) Slice through a subtomogram average of the Cx43 lattice is shown. One channel is indicated (arrowhead). Scale bar, 10 nm for (A) to (C). ( B ) Slice through the subtomogram average, along the solid line in (A). ( C ) Slice along the dashed line in (A). Channels are indicated in (B) and (C) (arrowheads). ( D ) Isosurface of a subtomogram average. A low-pass filter to 20-Å resolution has been applied to improve the interpretability of the lateral layers. Cx43 atomic models (PDB: 7Z22, residues 17 to 105 and 151 to 235) are shown in green. Two hemichannels forming a full channel are indicated (arrowheads). The convex (+) and concave (−) sides of the lattice are indicated in (B) to (D). ( E ) Density distribution as a function of distance from the lattice midpoint is shown. Density is in arbitrary units. The inner (IL) and outer (OL) leaflets of the two lipid bilayers are marked. The regions corresponding to intracellular density bridging Cx43 hexamers, denoted as lateral-contact layers (LCLs), are labeled. An additional layer of density on the concave side of the gap junction is indicated with an asterisk. ( F to H ) Isosurface renderings are shown from the top (+ to – direction) for the extracellular region (gap), inner leaflet (IL1), and intracellular densities (OL1). ( I ) Rendering of the CG MD simulation setup. The connexins are green, the POPC lipids are blue (head groups in a darker shade), and cholesterol is magenta. The solvent water is rendered as a transparent cube. The inset shows the area indicated. ( J and K ) Number of POPC lipids (J) and cholesterol molecules (K) around the centermost gap junction hemichannel is plotted as a function of the simulation time for both the membranes (+ and – sides).

Figure Legend Snippet: ( A ) Slice through a subtomogram average of the Cx43 lattice is shown. One channel is indicated (arrowhead). Scale bar, 10 nm for (A) to (C). ( B ) Slice through the subtomogram average, along the solid line in (A). ( C ) Slice along the dashed line in (A). Channels are indicated in (B) and (C) (arrowheads). ( D ) Isosurface of a subtomogram average. A low-pass filter to 20-Å resolution has been applied to improve the interpretability of the lateral layers. Cx43 atomic models (PDB: 7Z22, residues 17 to 105 and 151 to 235) are shown in green. Two hemichannels forming a full channel are indicated (arrowheads). The convex (+) and concave (−) sides of the lattice are indicated in (B) to (D). ( E ) Density distribution as a function of distance from the lattice midpoint is shown. Density is in arbitrary units. The inner (IL) and outer (OL) leaflets of the two lipid bilayers are marked. The regions corresponding to intracellular density bridging Cx43 hexamers, denoted as lateral-contact layers (LCLs), are labeled. An additional layer of density on the concave side of the gap junction is indicated with an asterisk. ( F to H ) Isosurface renderings are shown from the top (+ to – direction) for the extracellular region (gap), inner leaflet (IL1), and intracellular densities (OL1). ( I ) Rendering of the CG MD simulation setup. The connexins are green, the POPC lipids are blue (head groups in a darker shade), and cholesterol is magenta. The solvent water is rendered as a transparent cube. The inset shows the area indicated. ( J and K ) Number of POPC lipids (J) and cholesterol molecules (K) around the centermost gap junction hemichannel is plotted as a function of the simulation time for both the membranes (+ and – sides).

Techniques Used: Labeling, Solvent

( A to C ) Isosurface renderings of the cryo-ET subtomogram average at 14-Å resolution segmented with a cylindrical mask are shown as gray transparent surfaces, together with a partial atomic model of the Cx43 channel (PDB: 7Z22, residues 17 to 105 and 151 to 235). The inset indicates the level of the cross sections taken from the middle of the top bilayer (A), the middle of the extracellular loops (B), and the middle of the bottom bilayer (C). The TM helices are numbered 1 to 4 for one Cx monomer in both hemichannels. The convex (+) and concave (−) sides of the gap junction are indicated. ( D ) Close-up of the hemichannel cryo-ET map (this study) and a single-particle cryo-EM structure of the Cx43 hemichannel (EMD-14475), low-pass filtered to the same resolution (14 Å) for comparison. At this resolution, the N-terminal helices (residues 1 to 16, purple) closing the channel are visible in the cryo-EM density. The dashed lines indicate the position of the two membrane leaflets in both structures. ( E and F ) Close-ups of the intracellular areas indicated in the inset (dashed rectangles) are shown. In both, a stalk-like density can be seen, connecting the density below it (stem) and the density above it [lateral contacts layer (LCL)].

Figure Legend Snippet: ( A to C ) Isosurface renderings of the cryo-ET subtomogram average at 14-Å resolution segmented with a cylindrical mask are shown as gray transparent surfaces, together with a partial atomic model of the Cx43 channel (PDB: 7Z22, residues 17 to 105 and 151 to 235). The inset indicates the level of the cross sections taken from the middle of the top bilayer (A), the middle of the extracellular loops (B), and the middle of the bottom bilayer (C). The TM helices are numbered 1 to 4 for one Cx monomer in both hemichannels. The convex (+) and concave (−) sides of the gap junction are indicated. ( D ) Close-up of the hemichannel cryo-ET map (this study) and a single-particle cryo-EM structure of the Cx43 hemichannel (EMD-14475), low-pass filtered to the same resolution (14 Å) for comparison. At this resolution, the N-terminal helices (residues 1 to 16, purple) closing the channel are visible in the cryo-EM density. The dashed lines indicate the position of the two membrane leaflets in both structures. ( E and F ) Close-ups of the intracellular areas indicated in the inset (dashed rectangles) are shown. In both, a stalk-like density can be seen, connecting the density below it (stem) and the density above it [lateral contacts layer (LCL)].

Techniques Used: Tomography, Single Particle, Cryo-EM Sample Prep, Comparison, Membrane

Image Search Results

Journal: Science Advances

Article Title: In situ structure of the human gap junction

doi: 10.1126/sciadv.aea4183

Figure Lengend Snippet: ( A ) A schematic representation of the gap junction assembly from connexin 43 (Cx) monomers to hemichannels and to the gap junction plaque between two cells. Intra- (IL) and extracellular (EL) loops are labeled. The transmembrane (TM) helices are numbered 1 to 4. ( B ) One-nm-thick slice through a cryo-ET reconstruction of a cell-cell junction. Areas harboring Cx43 channels (green), 80 S ribosomes (cyan), and microtubules (pink) have been indicated. A mitochondrion has been labeled (M). Scale bars, 100 nm. ( C ) Close-up of the area indicated in (B). Side views of Cx43 GJCs bridging two plasma membranes are indicated with arrowheads. Scale bar, 10 nm. ( D ) Close-up from a different cryo-ET reconstruction showing top views of the Cx43 GJCs. Scale bar, 10 nm. ( E ) Results of template matching for Cx43 GJCs, 80 S ribosomes, and microtubule segments are shown after plotting the templates back onto the cryo-ET tomogram (illustrated with a 1-nm-thick slice in the background). The gap junction is depicted as a transparent surface (green) fitted to the Cx43 channel positions for visual clarity. ( F ) Histogram of pairwise distances between 80 S ribosomes and microtubule segments (MTs) to the Cx43 GJCs, calculated from multiple cryo-ET reconstructions ( n = 26). The 15-nm-wide zone occupied by Cx43 channels is indicated in green. The 22-nm-wide ribosome exclusion zone (REZ) is indicated in gray.

Article Snippet: The cryo-ET map of the Cx43 lattice has been deposited in the Electron Microscopy DataBank (EMDB) with the deposition ID EMD-54024.

Techniques: Labeling, Tomography, Clinical Proteomics

Journal: Science Advances

Article Title: In situ structure of the human gap junction

doi: 10.1126/sciadv.aea4183

Figure Lengend Snippet: ( A ) Slice through a subtomogram average of the Cx43 lattice is shown. One channel is indicated (arrowhead). Scale bar, 10 nm for (A) to (C). ( B ) Slice through the subtomogram average, along the solid line in (A). ( C ) Slice along the dashed line in (A). Channels are indicated in (B) and (C) (arrowheads). ( D ) Isosurface of a subtomogram average. A low-pass filter to 20-Å resolution has been applied to improve the interpretability of the lateral layers. Cx43 atomic models (PDB: 7Z22, residues 17 to 105 and 151 to 235) are shown in green. Two hemichannels forming a full channel are indicated (arrowheads). The convex (+) and concave (−) sides of the lattice are indicated in (B) to (D). ( E ) Density distribution as a function of distance from the lattice midpoint is shown. Density is in arbitrary units. The inner (IL) and outer (OL) leaflets of the two lipid bilayers are marked. The regions corresponding to intracellular density bridging Cx43 hexamers, denoted as lateral-contact layers (LCLs), are labeled. An additional layer of density on the concave side of the gap junction is indicated with an asterisk. ( F to H ) Isosurface renderings are shown from the top (+ to – direction) for the extracellular region (gap), inner leaflet (IL1), and intracellular densities (OL1). ( I ) Rendering of the CG MD simulation setup. The connexins are green, the POPC lipids are blue (head groups in a darker shade), and cholesterol is magenta. The solvent water is rendered as a transparent cube. The inset shows the area indicated. ( J and K ) Number of POPC lipids (J) and cholesterol molecules (K) around the centermost gap junction hemichannel is plotted as a function of the simulation time for both the membranes (+ and – sides).

Article Snippet: The cryo-ET map of the Cx43 lattice has been deposited in the Electron Microscopy DataBank (EMDB) with the deposition ID EMD-54024.

Techniques: Labeling, Solvent

Journal: Science Advances

Article Title: In situ structure of the human gap junction

doi: 10.1126/sciadv.aea4183

Figure Lengend Snippet: ( A to C ) Isosurface renderings of the cryo-ET subtomogram average at 14-Å resolution segmented with a cylindrical mask are shown as gray transparent surfaces, together with a partial atomic model of the Cx43 channel (PDB: 7Z22, residues 17 to 105 and 151 to 235). The inset indicates the level of the cross sections taken from the middle of the top bilayer (A), the middle of the extracellular loops (B), and the middle of the bottom bilayer (C). The TM helices are numbered 1 to 4 for one Cx monomer in both hemichannels. The convex (+) and concave (−) sides of the gap junction are indicated. ( D ) Close-up of the hemichannel cryo-ET map (this study) and a single-particle cryo-EM structure of the Cx43 hemichannel (EMD-14475), low-pass filtered to the same resolution (14 Å) for comparison. At this resolution, the N-terminal helices (residues 1 to 16, purple) closing the channel are visible in the cryo-EM density. The dashed lines indicate the position of the two membrane leaflets in both structures. ( E and F ) Close-ups of the intracellular areas indicated in the inset (dashed rectangles) are shown. In both, a stalk-like density can be seen, connecting the density below it (stem) and the density above it [lateral contacts layer (LCL)].

Article Snippet: The cryo-ET map of the Cx43 lattice has been deposited in the Electron Microscopy DataBank (EMDB) with the deposition ID EMD-54024.

Techniques: Tomography, Single Particle, Cryo-EM Sample Prep, Comparison, Membrane

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